Protein_Domain
FLO1

Part:BBa_K2407004:Design

Designed by: Xinyue Cao   Group: iGEM17_Tianjin   (2017-10-12)


FLO1 gene triggers the yeast flocculation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 952
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4336
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
    Illegal AgeI site found at 850
    Illegal AgeI site found at 892
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1297
    Illegal AgeI site found at 1432
    Illegal AgeI site found at 1567
    Illegal AgeI site found at 1702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1189
    Illegal BsaI.rc site found at 1324
    Illegal BsaI.rc site found at 1459
    Illegal BsaI.rc site found at 1594
    Illegal BsaI.rc site found at 2134


Design Notes

we use overlap to link promoter, FLO1 gene and terminator so we need to learn to design the overlap primers.


Source

yeast chromosome 1

References